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RIKEN BioResource Research Center (BRC®) Next Generation Human Disease Model Research Team

Team Director: Takanori Amano (Ph.D.)

Research Summary

Takanori  Amano(Ph.D.)

For development of mouse models which recapitulate pathology of intractable diseases, aging-associated diseases and lifestyle diseases, the genome-editing technology is used to produce knock-in point and multiple mutant mice based on genomic information of patients. The developed mice are analyzed and evaluated through the standard platform built by the International Mouse Phenotyping Consortium. We collaborate with external clinical experts for disease mechanisms and pharmacokinetics to establish POC (proof-of-concept) in preclinical studies. The disease models together with associated information useful for diagnosis, therapies and drug discovery will be distributed to the biomedical research community.

Main Research Fields

  • Biology

Related Research Fields

  • Biological Sciences
  • Medicine, Dentistry & Pharmacy
  • Genetics/Chromosome dynamics
  • Developmental biology
  • Human genetics

Keywords

  • disease model
  • mouse genetics
  • genome
  • variation

Selected Publications

  • 1. Yoshida K, Takada T, Muratani M and Amano T.
    "Allele-specific gene expression in F1 hybrid mice reveals structural variants affecting macrophage characteristics."
    Scientific Reports, 15, 1, 37846. (2025).
  • 2. Dinh TTH, Imura C, Shiokawa M, Ayabe S, Yoshiki A, Inoue H and Amano T.
    "A partial deletion of the Tardbp 3´UTR affects TDP-43 regulation and leads to motor dysfunction in mice"
    Experimental Animals 75, 1, 25-61. (2025).
  • 3. Hang A, Shao A, Shea M, Roux MJ, Imai-Leonard DM, Adams DJ, Amano T, Amarie OV, Berberovic Z, Bour R, Bower L, Leonard BC, Brown SD, Cho SY, Clementson-Mobbs S, D’Souza AJ, Dickinson M, Eskandarian M, Flenniken AM, Fuchs H, Gailus-Durner V, Heaney J, Hérault Y, Hrabe de Angelis M, Hsu CW, Jin S, Joynson R, Kang YK, Kim H, Masuya H, Nam KH, Noh H, Nutter LMJ, Palkova M, Prochazka J, Raishbrook MJ, Riet F, Salazar J, Seavitt JR, Sedlacek R, Selloum M, Seo KY, Seong JK, Shin HS, Shiroishi T, Sorg T, Stewart M, Tamura M, Tolentino H, Udensi U, Wells S, Wurst W, Yoshiki A, Meziane H, Yiu G, Sieving PA, Lanoue L, Lloyd KCK, McKerlie C and Moshiri A; International Mouse Phenotyping Consortium (IMPC).
    "Ocular Phenotyping of Knockout Mice Identifies Genes Associated With Late Adult Retinal Phenotypes."
    Investigative Ophthalmology & Visual Science, 66, 6, 64. (2025).
  • 4. Rahmawati NY, Dinh TTH, Oikawa T, Shinogi A, Ikeda K, Kadota M, Tamura M, Amano T and Yoshiki A.
    "Luminescent mouse model of endometriosis: three-dimensional morphology of lesions and cytokine profiles"
    Experimental Animals 74, 4, 419-428. (2025).
  • 5. Briere A, Vo P, Yang B, Adams D, Amano T, Amarie O, Berberovic Z, Bower L, Brown SDM, Burrill S, Cho SY, Clementson-Mobbs S, D’souza A, Eskandarian M, Flenniken AM, Fuchs H, Gailus-Durner V, Hérault Y, Hrabe de Angelis M, Jin S, Joynson R, Kang YK, Kim H, Masuya H, Meziane H, Nam KH, Noh H, Nutter LMJ, Palkova M, Prochazka J, Raishbrook MJ, Riet F, Salazar J, Sedlacek R, Selloum M, Seo KY, Seong JK, Shin HS, Shiroishi T, Stewart M, Svenson K, Tamura M, Tolentino H, Wells S, Wurst W, Yoshiki A, Lanoue L, Lloyd KCK, Leonard BC, Roux MJ, McKerlie C and Moshiri A; International Mouse Phenotyping Consortium.
    "Systematic Ocular Phenotyping of Knockout Mouse Lines Identifies Genes Associated With Age-Related Corneal Dystrophies"
    Investigative Ophthalmology & Visual Science, 66, 5, 7. (2025).
  • 6. Vo P, Imai-Leonard DM, Yang B, Briere A, Shao A, Casanova MI, Adams D, Amano T, Amarie O, Berberovic Z, Bower L, Braun R, Brown S, Burrill S, Cho SY, Clementson-Mobbs S, D’Souza A, Dickinson M, Eskandarian M, Flenniken AM, Fuchs H, Gailus-Durner V, Heaney J, Hérault Y, Angelis MH, Hsu CW, Jin S, Joynson R, Kang YK, Kim H, Masuya H, Meziane H, Murray S, Nam KH, Noh H, Nutter LMJ, Palkova M, Prochazka J, Raishbrook MJ, Riet F, Ryan J, Salazar J, Seavey Z, Seavitt JR, Sedlacek R, Selloum M, Seo KY, Seong JK, Shin HS, Shiroishi T, Stewart M, Svenson K, Tamura M, Tolentino H, Udensi U, Wells S, White J, Willett A, Wotton J, Wurst W, Yoshiki A; International Mouse Phenotyping Consortium; Lanoue L, Lloyd KCK, Leonard BC, Roux MJ, McKerlie C and Moshiri A.
    "Systematic ocular phenotyping of 8,707 knockout mouse lines identifies genes associated with abnormal corneal phenotypes"
    EBMC Genomics 26, 1, 48. (2025).
  • 7. Hayashi A, Kamio K, Miyanaga A, Yoshida K, Noro R, Matsuda K, Tozuka T,Omori M, Hirao M, Fukuizumi A, Hisakane K, Takeuchi S, Matsumoto M, Kasahara K, Amano T, Honda K and Seike M.
    "Ivermectin Enhances Paclitaxel Efficacy by Overcoming Resistance Through Modulation of ABCB1 in Non-small Cell Lung Cancer"
    Anticancer Research 44, (12) 5271-5282. (2024).
  • 8. Saito A, Tahara R, Hirose M, Kadota M, Hasegawa A, Kondo S, Kato H, Amano T, Yoshiki A, Ogura A and Kiyosawa H.
    "Inter-subspecies mouse F1 hybrid embryonic stem cell lines newly established for studies of allelic imbalance in gene expression"
    Experimental Animals 24-0002. (2024).
  • 9. Kawashima H, Nishimata S, Shinji S, Morishima Y, Tsutsumi N, Kashiwagi K, Amano T, Tamura M, Ayabe S and Nakashima K.
    "Three Siblings with Aarskog Scott Syndrome Together with Mental Retardation and Giant Megacolon"
    Glob. J. Pedia. 2, 1, IFN1000278. (2024).
  • 10. Tada R+, Higashidate T+, Amano T, Ishikawa S, Yokoyama C, Kobari S, Nara S, Ishida K, Kawaguchi A, Ochi H, Ogino H, Yakushiji-Kaminatsui N, Sakamoto J, Kamei Y, Tamura K and Yokoyama A.
    "The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis"
    Developmental Biology 500, 22-30. (2023).
    (+Authors equally contributed.)

Lab Members

Principal investigator

Takanori Amano
Team Director

Core members

Tra Thi Huong Dinh
Research Scientist
Ayaka Saito
Postdoctoral Researcher
Chigusa Imura
Technical Staff II
Mayu Shiokawa
Technical Staff II

Contact Information

3-1-1 Koyadai, Tsukuba,
Ibaraki, 305-0074

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