RIKEN Center for Brain Science Laboratory for Imaging Neural Dynamics
Team Director: Shigeo Okabe (M.D., Ph.D.)
Research Summary
Our team aims to analyze the processes of formation, maintenance, and dysfunction of neural circuits underlying brain function using advanced microscopy techniques that have rapidly developed in recent years, and to achieve an integrated understanding of the processes from molecular mechanisms to the emergence of circuit function. To this end, we will advance morphological approaches, including super-resolution microscopy, correlative light and electron microscopy (CLEM), and volume electron microscopy. By applying quantitative, reproducible analytical frameworks grounded in the mathematical sciences, we will elucidate the diversity of synaptic connectivity within circuits and their functional relationships with glial cells.
Main Research Fields
- Medicine, Dentistry & Pharmacy
Related Research Fields
- Biology
- Neuroscience and related fields / Anatomy and histopathology of nervous system-related
Keywords
- Synapse
- Neural circuit
- Imaging
- Glia
- Neuropsychiatric disorders
Selected Publications
Papers with an asterisk(*) are based on research conducted outside of RIKEN.
- 1.
*Kamei R. and Okabe S.:
"In vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia"
Glia, 71, 2005-2023. (2023). - 2.
*Obashi, K., Matsuda, A., Inoue, Y., and Okabe S.:
"Precise temporal regulation of molecular diffusion within dendritic spines by actin polymers during structural plasticity"
Cell Reports 27, 1503-1515, (2019). - 3.
*Kashiwagi, Y., Higashi, T., Obashi, K., Sato, Y., Komiyama, N., Grant, S. G. N. and Okabe S.:
"Computational geometry analysis of dendritic spines by structured illumination microscopy"
Nature Communications 10, 1285, (2019). - 4.
*Urata, S., Iida, T., Yamamoto, M., Mizushima, Y., Fujimoto, C., Matsumoto, Y., Yamasoba, T. and Okabe S.:
"Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning"
eLIFE, 8, pii: e40946, (2019). - 5.
*Higashi T, Tanaka S, Iida T, and Okabe S.:
"Synapse elimination triggered by BMP4 exocytosis and presynaptic BMP receptor activation"
Cell Reports 22, 919-929, (2018). - 6.
*Isshiki, M., Tanaka, S., Kuriu, T., Tabuchi, K., Takumi, T. and Okabe S.:
"Enhanced synapse remodelling as a common phenotype in mouse models of autism"
Nature Communications 5, 4742, (2014). - 7.
*Shin, E., Kashiwagi, Y., Kuriu, T., Iwasaki, H., Tanaka, T., Koizumi, H., Gleeson, J. G. and Okabe S.:
"Doublecortin-like kinase enhances dendritic remodeling and negatively regulates synapse maturation"
Nature Communications 4, 1440, (2013) - 8.
*Ito-Ishida, A., Miyazaki, T., Miura, E., Matsuda, K., Watanabe, M., Yuzaki, M and Okabe S.:
"Presynaptically released Cbln1 induces dynamic axonal structural changes by interacting with GluD2 during cerebellar synapse formation"
Neuron 76, 549-564, (2012). - 9.
*Kawabata, I., Kashiwagi, Y., Obashi, K., Ohkura, M., Nakai, J., Wynshaw-Boris, A., Yanagawa, Y., and Okabe S.:
"LIS1-dependent retrograde translocation of excitatory synapses in developing interneuron dendrites"
Nature Communications 3, 722, (2012).
Lab Members
Principal investigator
- Shigeo Okabe
- Team Director
Contact Information
Brain Science Central Building,
2-1 Hirosawa Wako City, Saitama 351-0198
Email: shigeo.okabe@riken.jp